Analysis Note

ControlNIH/3T3 cell lysate

Application

Research CategoryStem Cell Research

Anti-BMP Antibody15 is a highly specific rabbit polyclonal antibody, that targets BMP & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected BMP15 in NIH/3T3 cells.

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bone morphogenetic protein 15 (BMP15), also known as Growth/differentiation factor 9B (GDF9B), is part of the transforming growth factor-beta (TGF-β) superfamily. BMP15 is an oocyte-specific growth and differentiation factor that stimulates folliculogenesis and development as well as granulosa cell (GC) growth. BMP15 is expressed exclusively in the oocyte. BMP15 defects are linked to ovarian dysgenesis type 2 (ODG2) and premature ovarian failure type 4 (POF4). Potentially, BMP15 could be an oogenesis marker, used to monitor the differentiation of human amniotic fluid stem cells into oocyte-like cells.

Immunogen

KLH-conjugated linear peptide corresponding to human BMP15.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected BMP15 in 15 µg of NIH/3T3 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~53 kDa observed. Uncharacterized bands may appear in some lysate(s).